Poles apart: prokaryotic polar organelles and their spatial regulation.

Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells.

The spindle pole localization of gamma-tubulin was compared in wild type and acentriolar cultured Drosophila cells using polyclonal antibodies specifically raised against the carboxy terminal amino acid sequence of Drosophila gamma-tubulin-1 (-KSEDSRSVTSAGS). During interphase, gamma-tubulin was present in the centrosome of wild type cells and accumulated around this organelle in a cell cycle d...

An Intracellular Nanotrap Redirects Proteins and Organelles in Live Bacteria

UNLABELLED Owing to their small size and enhanced stability, nanobodies derived from camelids have previously been used for the construction of intracellular "nanotraps," which enable redirection and manipulation of green fluorescent protein (GFP)-tagged targets within living plant and animal cells. By taking advantage of intracellular compartmentalization in the magnetic bacterium Magnetospiri...

Modeling Spatial Distribution of Intracellular Organelles by Molecular Motors

Polar Flagellar Biosynthesis and a Regulator of Flagellar Number Influence Spatial Parameters of Cell Division in Campylobacter jejuni

Spatial and numerical regulation of flagellar biosynthesis results in different flagellation patterns specific for each bacterial species. Campylobacter jejuni produces amphitrichous (bipolar) flagella to result in a single flagellum at both poles. These flagella confer swimming motility and a distinctive darting motility necessary for infection of humans to cause diarrheal disease and animals ...

Bipartite Polar Classification for Surface Reconstruction

In this paper, we propose bipartite polar classification to augment an input unorganized point set P with two disjoint groups of points distributed around the ambient space of P to assist the task of surface reconstruction. The goal of bipartite polar classification is to obtain a space partitioning of P by assigning pairs of Voronoi poles into two mutually invisible sets lying in the opposite ...